产品货号：
GS4486
中文名称：
重组人IL-33
英文名称：
Recombinant Human Interleukin-33
产品规格：
2μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 17.9kDa,a single non-glycosylated polypeptide chain containing 159 amino acids.
储存条件：冷冻（-20℃）
概述
IL-33,encoded by IL-33 gene located on the Chr.9 in humans,is a 30 kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20%a.a.sequence identity with other members.IL-33 secreted by high endothelial venules at high levels,which is found in tonsils,peyer patches and mesenteric lymph nodes,but not in placenta.It is upregulated in arterial smooth muscle,dermal fibroblasts,and keratinocytes following IL1α or IL1β stimulation.It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88,IRAK1,IRAK4,and TRAF6,followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2,MAPK14,and MAPK8.IL-33 mature protein has 52-58%a.a.sequence identity with mouse and rat IL-33.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人IL-33，IL-33, Human，Recombinant Human Interleukin-33
储存条件：冷冻（-20℃）
概述
IL-33,encoded by IL-33 gene located on the Chr.9 in humans,is a 30 kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20%a.a.sequence identity with other members.IL-33 secreted by high endothelial venules at high levels,which is found in tonsils,peyer patches and mesenteric lymph nodes,but not in placenta.It is upregulated in arterial smooth muscle,dermal fibroblasts,and keratinocytes following IL1α or IL1β stimulation.It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88,IRAK1,IRAK4,and TRAF6,followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2,MAPK14,and MAPK8.IL-33 mature protein has 52-58%a.a.sequence identity with mouse and rat IL-33.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞97%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05ng/ml,corresponding to a specific activity of>2.0×107 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,150 mM NaCl,1mM EDTA,pH7.4. |
| 内毒素 | Less than 1 EU/μg of rHuIL-33 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | SITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVLLSYYESQHPS NE SGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEKPLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET |
相关搜索：重组人IL-33，IL-33, Human，Recombinant Human Interleukin-33